Formation and Dissociation of Sperm Bundles in Monotremes.
Identifieur interne : 001E78 ( Main/Exploration ); précédent : 001E77; suivant : 001E79Formation and Dissociation of Sperm Bundles in Monotremes.
Auteurs : Brett Nixon [Australie] ; Heath Ecroyd [Australie] ; Jean-Louis Dacheux [France] ; Francoise Dacheux [France] ; Valerie Labas [France] ; Steve D. Johnston [Australie] ; Russell C. Jones [Australie]Source :
- Biology of reproduction [ 1529-7268 ] ; 2016.
Descripteurs français
- KwdFr :
- Adhérence cellulaire (physiologie), Animaux, Capacitation des spermatozoïdes (physiologie), Echidna (anatomie et histologie), Echidna (physiologie), Femelle, Fécondation (physiologie), Interaction sperme-ovule (physiologie), Maturation du spermatozoïde (physiologie), Motilité des spermatozoïdes (physiologie), Mâle, Ostéonectine (physiologie), Platypus (anatomie et histologie), Platypus (physiologie), Poulets, Protéome (isolement et purification), Protéome (métabolisme), Réaction acrosomique (physiologie), Spermatozoïdes (cytologie), Spermatozoïdes (physiologie), Spécificité d'espèce, Épididyme (anatomie et histologie), Épididyme (physiologie).
- MESH :
- anatomie et histologie : Echidna, Platypus, Épididyme.
- cytologie : Spermatozoïdes.
- isolement et purification : Protéome.
- métabolisme : Protéome.
- physiologie : Adhérence cellulaire, Capacitation des spermatozoïdes, Echidna, Fécondation, Interaction sperme-ovule, Maturation du spermatozoïde, Motilité des spermatozoïdes, Ostéonectine, Platypus, Réaction acrosomique, Spermatozoïdes, Épididyme.
- Animaux, Femelle, Mâle, Poulets, Spécificité d'espèce.
English descriptors
- KwdEn :
- Acrosome Reaction (physiology), Animals, Cell Adhesion (physiology), Chickens, Echidna (anatomy & histology), Echidna (physiology), Epididymis (anatomy & histology), Epididymis (physiology), Female, Fertilization (physiology), Male, Osteonectin (physiology), Platypus (anatomy & histology), Platypus (physiology), Proteome (isolation & purification), Proteome (metabolism), Species Specificity, Sperm Capacitation (physiology), Sperm Maturation (physiology), Sperm Motility (physiology), Sperm-Ovum Interactions (physiology), Spermatozoa (cytology), Spermatozoa (physiology).
- MESH :
- chemical , isolation & purification : Proteome.
- chemical , metabolism : Proteome.
- chemical , physiology : Osteonectin.
- anatomy & histology : Echidna, Epididymis, Platypus.
- cytology : Spermatozoa.
- physiology : Acrosome Reaction, Cell Adhesion, Echidna, Epididymis, Fertilization, Platypus, Sperm Capacitation, Sperm Maturation, Sperm Motility, Sperm-Ovum Interactions, Spermatozoa.
- Animals, Chickens, Female, Male, Species Specificity.
Abstract
Because monotremes are the earliest offshoot of the mammalian lineage, the platypus and short-beaked echidna were studied as model animals to assess the origin and biological significance of adaptations considered unique to therian mammals: epididymal sperm maturation and subsequent capacitation. We show that spermatozoa from both species assemble into bundles of approximately 100 cells during passage through the epididymis and that an epididymal protein-secreted protein, acidic, cysteine-rich (osteonectin; SPARC)-is involved in bundle formation. The bundles persisted during incubation in vitro for at least 1 h under conditions that capacitate therian spermatozoa, and then underwent a time-dependent dissociation to release spermatozoa capable of fertilization. Only after this dissociation could the spermatozoa bind to the perivitelline membrane of a hen's egg, display an altered form of motility reminiscent of hyperactivation, and be induced to undergo an acrosome reaction. It is concluded that the development of sperm bundles in the monotreme epididymis mandates that they require a time-dependent process to be capable of fertilizing an ovum. However, because this functional end point was achieved without overt changes in protein tyrosine phosphorylation (a hallmark of capacitation in therians), it is concluded that the process in monotremes is distinctly different from capacitation in therian mammals.
DOI: 10.1095/biolreprod.116.140491
PubMed: 27557648
Affiliations:
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Johnston</name><affiliation wicri:level="1"><nlm:affiliation>School of Agriculture and Food Science, The University of Queensland, Gatton, Queensland, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of Agriculture and Food Science, The University of Queensland, Gatton, Queensland</wicri:regionArea><wicri:noRegion>Queensland</wicri:noRegion></affiliation></author><author><name sortKey="Jones, Russell C" sort="Jones, Russell C" uniqKey="Jones R" first="Russell C" last="Jones">Russell C. Jones</name><affiliation wicri:level="1"><nlm:affiliation>Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales</wicri:regionArea><wicri:noRegion>New South Wales</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27557648</idno><idno type="pmid">27557648</idno><idno type="doi">10.1095/biolreprod.116.140491</idno><idno type="wicri:Area/PubMed/Corpus">001782</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001782</idno><idno type="wicri:Area/PubMed/Curation">001759</idno><idno type="wicri:explorRef" 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Brett.Nixon@newcastle.edu.au.</nlm:affiliation><country wicri:rule="url">Australie</country><wicri:regionArea>Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales</wicri:regionArea><wicri:noRegion>New South Wales</wicri:noRegion></affiliation></author><author><name sortKey="Ecroyd, Heath" sort="Ecroyd, Heath" uniqKey="Ecroyd H" first="Heath" last="Ecroyd">Heath Ecroyd</name><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences and Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of Biological Sciences and Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales</wicri:regionArea><wicri:noRegion>New South Wales</wicri:noRegion></affiliation></author><author><name sortKey="Dacheux, Jean Louis" sort="Dacheux, Jean Louis" uniqKey="Dacheux J" first="Jean-Louis" last="Dacheux">Jean-Louis Dacheux</name><affiliation wicri:level="1"><nlm:affiliation>Institut National de la Recherche Agronomique (INRA), Unités Mixtes de Recherches (UMR) 85 Physiologie de la Reproduction et des Comportements, Nouzilly, France Centre National de la Recherche Scientifique, UMR 7247, Nouzilly, France Université François Rabelais de Tours, Tours, France l'Institut Français du Cheval et de l'Équitation, Nouzilly, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut National de la Recherche Agronomique (INRA), Unités Mixtes de Recherches (UMR) 85 Physiologie de la Reproduction et des Comportements, Nouzilly, France Centre National de la Recherche Scientifique, UMR 7247, Nouzilly, France Université François Rabelais de Tours, Tours, France l'Institut Français du Cheval et de l'Équitation, Nouzilly</wicri:regionArea><wicri:noRegion>Nouzilly</wicri:noRegion><wicri:noRegion>Nouzilly</wicri:noRegion></affiliation></author><author><name sortKey="Dacheux, Francoise" sort="Dacheux, Francoise" uniqKey="Dacheux F" first="Francoise" last="Dacheux">Francoise Dacheux</name><affiliation wicri:level="1"><nlm:affiliation>INRA, Plateforme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, Nouzilly, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Plateforme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, Nouzilly</wicri:regionArea><wicri:noRegion>Nouzilly</wicri:noRegion><wicri:noRegion>Nouzilly</wicri:noRegion></affiliation></author><author><name sortKey="Labas, Valerie" sort="Labas, Valerie" uniqKey="Labas V" first="Valerie" last="Labas">Valerie Labas</name><affiliation wicri:level="1"><nlm:affiliation>Institut National de la Recherche Agronomique (INRA), Unités Mixtes de Recherches (UMR) 85 Physiologie de la Reproduction et des Comportements, Nouzilly, France Centre National de la Recherche Scientifique, UMR 7247, Nouzilly, France Université François Rabelais de Tours, Tours, France l'Institut Français du Cheval et de l'Équitation, Nouzilly, France INRA, Plateforme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, Nouzilly, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut National de la Recherche Agronomique (INRA), Unités Mixtes de Recherches (UMR) 85 Physiologie de la Reproduction et des Comportements, Nouzilly, France Centre National de la Recherche Scientifique, UMR 7247, Nouzilly, France Université François Rabelais de Tours, Tours, France l'Institut Français du Cheval et de l'Équitation, Nouzilly, France INRA, Plateforme d'Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, Nouzilly</wicri:regionArea><wicri:noRegion>Nouzilly</wicri:noRegion><wicri:noRegion>Nouzilly</wicri:noRegion></affiliation></author><author><name sortKey="Johnston, Steve D" sort="Johnston, Steve D" uniqKey="Johnston S" first="Steve D" last="Johnston">Steve D. Johnston</name><affiliation wicri:level="1"><nlm:affiliation>School of Agriculture and Food Science, The University of Queensland, Gatton, Queensland, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of Agriculture and Food Science, The University of Queensland, Gatton, Queensland</wicri:regionArea><wicri:noRegion>Queensland</wicri:noRegion></affiliation></author><author><name sortKey="Jones, Russell C" sort="Jones, Russell C" uniqKey="Jones R" first="Russell C" last="Jones">Russell C. Jones</name><affiliation wicri:level="1"><nlm:affiliation>Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales</wicri:regionArea><wicri:noRegion>New South Wales</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biology of reproduction</title><idno type="eISSN">1529-7268</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acrosome Reaction (physiology)</term><term>Animals</term><term>Cell Adhesion (physiology)</term><term>Chickens</term><term>Echidna (anatomy & histology)</term><term>Echidna (physiology)</term><term>Epididymis (anatomy & histology)</term><term>Epididymis (physiology)</term><term>Female</term><term>Fertilization (physiology)</term><term>Male</term><term>Osteonectin (physiology)</term><term>Platypus (anatomy & histology)</term><term>Platypus (physiology)</term><term>Proteome (isolation & purification)</term><term>Proteome (metabolism)</term><term>Species Specificity</term><term>Sperm Capacitation (physiology)</term><term>Sperm Maturation (physiology)</term><term>Sperm Motility (physiology)</term><term>Sperm-Ovum Interactions (physiology)</term><term>Spermatozoa (cytology)</term><term>Spermatozoa (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adhérence cellulaire (physiologie)</term><term>Animaux</term><term>Capacitation des spermatozoïdes (physiologie)</term><term>Echidna (anatomie et histologie)</term><term>Echidna (physiologie)</term><term>Femelle</term><term>Fécondation (physiologie)</term><term>Interaction sperme-ovule (physiologie)</term><term>Maturation du spermatozoïde (physiologie)</term><term>Motilité des spermatozoïdes (physiologie)</term><term>Mâle</term><term>Ostéonectine (physiologie)</term><term>Platypus (anatomie et histologie)</term><term>Platypus (physiologie)</term><term>Poulets</term><term>Protéome (isolement et purification)</term><term>Protéome (métabolisme)</term><term>Réaction acrosomique (physiologie)</term><term>Spermatozoïdes (cytologie)</term><term>Spermatozoïdes (physiologie)</term><term>Spécificité d'espèce</term><term>Épididyme (anatomie et histologie)</term><term>Épididyme (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Proteome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Proteome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Osteonectin</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Echidna</term><term>Platypus</term><term>Épididyme</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Echidna</term><term>Epididymis</term><term>Platypus</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Spermatozoïdes</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Adhérence cellulaire</term><term>Capacitation des spermatozoïdes</term><term>Echidna</term><term>Fécondation</term><term>Interaction sperme-ovule</term><term>Maturation du spermatozoïde</term><term>Motilité des spermatozoïdes</term><term>Ostéonectine</term><term>Platypus</term><term>Réaction acrosomique</term><term>Spermatozoïdes</term><term>Épididyme</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Acrosome Reaction</term><term>Cell Adhesion</term><term>Echidna</term><term>Epididymis</term><term>Fertilization</term><term>Platypus</term><term>Sperm Capacitation</term><term>Sperm Maturation</term><term>Sperm Motility</term><term>Sperm-Ovum Interactions</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chickens</term><term>Female</term><term>Male</term><term>Species Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Mâle</term><term>Poulets</term><term>Spécificité d'espèce</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Because monotremes are the earliest offshoot of the mammalian lineage, the platypus and short-beaked echidna were studied as model animals to assess the origin and biological significance of adaptations considered unique to therian mammals: epididymal sperm maturation and subsequent capacitation. We show that spermatozoa from both species assemble into bundles of approximately 100 cells during passage through the epididymis and that an epididymal protein-secreted protein, acidic, cysteine-rich (osteonectin; SPARC)-is involved in bundle formation. The bundles persisted during incubation in vitro for at least 1 h under conditions that capacitate therian spermatozoa, and then underwent a time-dependent dissociation to release spermatozoa capable of fertilization. Only after this dissociation could the spermatozoa bind to the perivitelline membrane of a hen's egg, display an altered form of motility reminiscent of hyperactivation, and be induced to undergo an acrosome reaction. It is concluded that the development of sperm bundles in the monotreme epididymis mandates that they require a time-dependent process to be capable of fertilizing an ovum. However, because this functional end point was achieved without overt changes in protein tyrosine phosphorylation (a hallmark of capacitation in therians), it is concluded that the process in monotremes is distinctly different from capacitation in therian mammals.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Nixon, Brett" sort="Nixon, Brett" uniqKey="Nixon B" first="Brett" last="Nixon">Brett Nixon</name></noRegion><name sortKey="Ecroyd, Heath" sort="Ecroyd, Heath" uniqKey="Ecroyd H" first="Heath" last="Ecroyd">Heath Ecroyd</name><name sortKey="Johnston, Steve D" sort="Johnston, Steve D" uniqKey="Johnston S" first="Steve D" last="Johnston">Steve D. Johnston</name><name sortKey="Jones, Russell C" sort="Jones, Russell C" uniqKey="Jones R" first="Russell C" last="Jones">Russell C. Jones</name></country><country name="France"><noRegion><name sortKey="Dacheux, Jean Louis" sort="Dacheux, Jean Louis" uniqKey="Dacheux J" first="Jean-Louis" last="Dacheux">Jean-Louis Dacheux</name></noRegion><name sortKey="Dacheux, Francoise" sort="Dacheux, Francoise" uniqKey="Dacheux F" first="Francoise" last="Dacheux">Francoise Dacheux</name><name sortKey="Labas, Valerie" sort="Labas, Valerie" uniqKey="Labas V" first="Valerie" last="Labas">Valerie Labas</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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